rabbit α suv3 Search Results


rabbit anti suv3  (Fortis Life Sciences)
93
Fortis Life Sciences rabbit anti suv3
a, dsRNA-seq experimental approach. b, dsRNA-seq reads across the mitochondrial genome spanning protein coding region in untreated HeLa cells. H-strand genes are shown as blue bars and L-strand as red bars. Short tRNA genes are denoted with T as the first letter. Data are representative of two experiments. c, Immunostaining of dsRNA in HeLa cells with anti-dsRNA (J2) antibody. Mitochondria and nuclei are stained with MitoTracker Deep Red and Hoechst, respectively. Scale bars, 10 μm. Graphs quantify co-localization of dsRNA foci with mitochondria. Data are mean ± s.d. from 29 cells. d, Anti-dsRNA (J2) staining in HeLa cells depleted for PNPase or <t>SUV3</t> by siRNA as in c. Different imaging settings were applied in panel c and d so that the J2 intensity of control cells varies. e, Western blot showing PNPase or SUV3 depletion. Blots are representative of four experiments. f, Quantification of dsRNA levels in PNPase- or SUV3-depleted cells. Data are mean ± s.d. from four experiments. For gel source data, see Supplementary Fig. 1.
Rabbit Anti Suv3, supplied by Fortis Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+%CE%B1+suv3/SUV3+Antibody/pmc06570621-456-28-31
Average 93 stars, based on 1 article reviews
rabbit anti suv3 - by Bioz Stars, 2026-11
93/100 stars
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rabbit anti suv3  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc rabbit anti suv3
a, dsRNA-seq experimental approach. b, dsRNA-seq reads across the mitochondrial genome spanning protein coding region in untreated HeLa cells. H-strand genes are shown as blue bars and L-strand as red bars. Short tRNA genes are denoted with T as the first letter. Data are representative of two experiments. c, Immunostaining of dsRNA in HeLa cells with anti-dsRNA (J2) antibody. Mitochondria and nuclei are stained with MitoTracker Deep Red and Hoechst, respectively. Scale bars, 10 μm. Graphs quantify co-localization of dsRNA foci with mitochondria. Data are mean ± s.d. from 29 cells. d, Anti-dsRNA (J2) staining in HeLa cells depleted for PNPase or <t>SUV3</t> by siRNA as in c. Different imaging settings were applied in panel c and d so that the J2 intensity of control cells varies. e, Western blot showing PNPase or SUV3 depletion. Blots are representative of four experiments. f, Quantification of dsRNA levels in PNPase- or SUV3-depleted cells. Data are mean ± s.d. from four experiments. For gel source data, see Supplementary Fig. 1.
Rabbit Anti Suv3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+%CE%B1+suv3/SimpleChIP+Human+c-Fos+Exon+3+Primers/pm30046113-826-42-58
Average 94 stars, based on 1 article reviews
rabbit anti suv3 - by Bioz Stars, 2026-11
94/100 stars
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suv39h2 rabbit polyclonal antibody  (OriGene)
N/A
Rabbit polyclonal anti SUV39H2 antibody
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rabbit anti-human suv39h2 polyclonal antibody  (Cusabio)
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Rabbit anti-Human SUV39H2 Polyclonal Antibody
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suv39h1 antibody, rabbit pab, antigen affinity purified  (Sino Biological)
N/A
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human SUV39H1, and purified by antigen affinity chromatography.
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rabbit anti-suv39h2 antibody  (RayBiotech inc)
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SUV39H2 Polyclonal Antibody
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suv39h1 polyclonal antibody  (Elabscience)
N/A
This gene encodes an evolutionarily conserved protein containing an N terminal chromodomain and a C terminal SET domain The encoded protein is a histone methyltransferase that trimethylates lysine 9 of histone H3 which results in
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rabbit anti-histone methyltransferase (suv39h1) antibody  (RayBiotech)
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Rabbit Anti-Histone Methyltransferase (SUV39H1) Antibody, (100 µg)
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suv39h2 antibody  (ProSci Incorporated)
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SUV39H2 Antibody raised in Rabbit validated in WB, IHC-P in Human, Mouse.
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suv39h2 antibody - middle region (arp48910_p050)  (Aviva Systems)
N/A
This is a rabbit polyclonal antibody against SUV39H2. It was validated on Western Blot using a cell lysate as a positive control. Aviva Systems Biology strives to provide antibodies covering each member of a whole
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suv39h2 rabbit pab  (ABclonal)
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Image Search Results


a, dsRNA-seq experimental approach. b, dsRNA-seq reads across the mitochondrial genome spanning protein coding region in untreated HeLa cells. H-strand genes are shown as blue bars and L-strand as red bars. Short tRNA genes are denoted with T as the first letter. Data are representative of two experiments. c, Immunostaining of dsRNA in HeLa cells with anti-dsRNA (J2) antibody. Mitochondria and nuclei are stained with MitoTracker Deep Red and Hoechst, respectively. Scale bars, 10 μm. Graphs quantify co-localization of dsRNA foci with mitochondria. Data are mean ± s.d. from 29 cells. d, Anti-dsRNA (J2) staining in HeLa cells depleted for PNPase or SUV3 by siRNA as in c. Different imaging settings were applied in panel c and d so that the J2 intensity of control cells varies. e, Western blot showing PNPase or SUV3 depletion. Blots are representative of four experiments. f, Quantification of dsRNA levels in PNPase- or SUV3-depleted cells. Data are mean ± s.d. from four experiments. For gel source data, see Supplementary Fig. 1.

Journal: Nature

Article Title: Mitochondrial double-stranded RNA triggers antiviral signalling in humans

doi: 10.1038/s41586-018-0363-0

Figure Lengend Snippet: a, dsRNA-seq experimental approach. b, dsRNA-seq reads across the mitochondrial genome spanning protein coding region in untreated HeLa cells. H-strand genes are shown as blue bars and L-strand as red bars. Short tRNA genes are denoted with T as the first letter. Data are representative of two experiments. c, Immunostaining of dsRNA in HeLa cells with anti-dsRNA (J2) antibody. Mitochondria and nuclei are stained with MitoTracker Deep Red and Hoechst, respectively. Scale bars, 10 μm. Graphs quantify co-localization of dsRNA foci with mitochondria. Data are mean ± s.d. from 29 cells. d, Anti-dsRNA (J2) staining in HeLa cells depleted for PNPase or SUV3 by siRNA as in c. Different imaging settings were applied in panel c and d so that the J2 intensity of control cells varies. e, Western blot showing PNPase or SUV3 depletion. Blots are representative of four experiments. f, Quantification of dsRNA levels in PNPase- or SUV3-depleted cells. Data are mean ± s.d. from four experiments. For gel source data, see Supplementary Fig. 1.

Article Snippet: The following antibodies were obtained commercially: mouse anti-ADAR1 mAb (sc-73408, Santa Cruz), rabbit anti-PNPT1 (ab96176, Abcam; sc-49315, Santa Cruz), mouse anti-dsRNA mAb J2 (10010500, Scions), anti-DNA (61014, Progen), rabbit anti-SUV3 (A303-055A, Bethyl Laboratories) 11 , mouse anti-RIG-I mAb (Alme-1) (AG-20B-0009, AdipoGen), rabbit anti-COX IV (3E11, Cell Signaling), rabbit anti-cytochrome c (NB100-91732, Novus Biologicals), rabbit anti-calnexin (2433, Cell Signaling), mouse anti-lamin A/C (4C11, Cell Signaling), donkey anti-mouse IgG (H+L) conjugated with Alexa Fluor 488 (A-21202, Lifetech), goat anti-mouse IgM conjugated with Alexa Fluor 555 (A-21426, Thermo Fisher Scientific), normal mouse IgG2a (sc-3878, Santa Cruz), mouse anti-MDA5 (in house from J.R.), rabbit anti-MAVS (ALX-210-929-C100, Enzo Life Sciences), rabbit anti-Bax (2772T, Cell Signaling), rabbit anti-Bak (6947T, Cell Signaling), rabbit anti-OXA1L (HPA003531, Sigma), mouse anti-α-Tubulin (T5168, Sigma), mouse anti-actin (ab8226, Abcam; A5441, Sigma), rabbit anti-Flag (PA1-984B, Thermo Fisher Scientific), HRP secondary anti-mouse (ab6728, Abcam; A9044, Sigma) 11 , HRP secondary anti-rabbit (A0545, Sigma; ab6721, Abcam) 11 , goat anti-mouse IgG (20 nm gold) preadsorbed (ab27242, Abcam).

Techniques: Immunostaining, Staining, Imaging, Western Blot

a, RT–qPCR analysis of IFNB1 expression in HeLa cells transfected with indicated siRNAs. Data are mean ± s.d. from three independent experiments. b, RT–qPCR analysis of Ifit1 expression in Ddx58+/− (control, RIG-I+), Ddx58−/− (RIG-I−) and Ifih1−/− (MDA5−) immortalized MEFs transfected with mtRNA, RIG-I (ppp-IVT-RNA99nt) or MDA5 (CIP-EMCV RNA) specific agonists. Data are the mean from two independent experiments. Values are plotted on a logarithmic scale. c, Left, RT–qPCR analysis of IFNB1 mRNA in HeLa cells treated with the indicated siRNAs. Data are mean from two independent experiments. Right, western blot of siRNA depletion efficiency. Blots are representative of two experiments. For gel source data, see Supplementary Fig. 1. d, Model of mtdsRNA suppression by the RNA degradosome. Loss of PNPase causes accumulation of mtdsRNA and release of mtdsRNA into the cytoplasm in a Bax–Bak dependent manner. PNPase restricts mtdsRNA in matrix (together with SUV3) and IMS. MDA5 acts as the primary mtdsRNA sensor transducing an interferon response through the MAVS signalling pathway.

Journal: Nature

Article Title: Mitochondrial double-stranded RNA triggers antiviral signalling in humans

doi: 10.1038/s41586-018-0363-0

Figure Lengend Snippet: a, RT–qPCR analysis of IFNB1 expression in HeLa cells transfected with indicated siRNAs. Data are mean ± s.d. from three independent experiments. b, RT–qPCR analysis of Ifit1 expression in Ddx58+/− (control, RIG-I+), Ddx58−/− (RIG-I−) and Ifih1−/− (MDA5−) immortalized MEFs transfected with mtRNA, RIG-I (ppp-IVT-RNA99nt) or MDA5 (CIP-EMCV RNA) specific agonists. Data are the mean from two independent experiments. Values are plotted on a logarithmic scale. c, Left, RT–qPCR analysis of IFNB1 mRNA in HeLa cells treated with the indicated siRNAs. Data are mean from two independent experiments. Right, western blot of siRNA depletion efficiency. Blots are representative of two experiments. For gel source data, see Supplementary Fig. 1. d, Model of mtdsRNA suppression by the RNA degradosome. Loss of PNPase causes accumulation of mtdsRNA and release of mtdsRNA into the cytoplasm in a Bax–Bak dependent manner. PNPase restricts mtdsRNA in matrix (together with SUV3) and IMS. MDA5 acts as the primary mtdsRNA sensor transducing an interferon response through the MAVS signalling pathway.

Article Snippet: The following antibodies were obtained commercially: mouse anti-ADAR1 mAb (sc-73408, Santa Cruz), rabbit anti-PNPT1 (ab96176, Abcam; sc-49315, Santa Cruz), mouse anti-dsRNA mAb J2 (10010500, Scions), anti-DNA (61014, Progen), rabbit anti-SUV3 (A303-055A, Bethyl Laboratories) 11 , mouse anti-RIG-I mAb (Alme-1) (AG-20B-0009, AdipoGen), rabbit anti-COX IV (3E11, Cell Signaling), rabbit anti-cytochrome c (NB100-91732, Novus Biologicals), rabbit anti-calnexin (2433, Cell Signaling), mouse anti-lamin A/C (4C11, Cell Signaling), donkey anti-mouse IgG (H+L) conjugated with Alexa Fluor 488 (A-21202, Lifetech), goat anti-mouse IgM conjugated with Alexa Fluor 555 (A-21426, Thermo Fisher Scientific), normal mouse IgG2a (sc-3878, Santa Cruz), mouse anti-MDA5 (in house from J.R.), rabbit anti-MAVS (ALX-210-929-C100, Enzo Life Sciences), rabbit anti-Bax (2772T, Cell Signaling), rabbit anti-Bak (6947T, Cell Signaling), rabbit anti-OXA1L (HPA003531, Sigma), mouse anti-α-Tubulin (T5168, Sigma), mouse anti-actin (ab8226, Abcam; A5441, Sigma), rabbit anti-Flag (PA1-984B, Thermo Fisher Scientific), HRP secondary anti-mouse (ab6728, Abcam; A9044, Sigma) 11 , HRP secondary anti-rabbit (A0545, Sigma; ab6721, Abcam) 11 , goat anti-mouse IgG (20 nm gold) preadsorbed (ab27242, Abcam).

Techniques: Quantitative RT-PCR, Expressing, Transfection, Western Blot

Oligonucleotide primers and siRNAs used in the study

Journal: Nature

Article Title: Mitochondrial double-stranded RNA triggers antiviral signalling in humans

doi: 10.1038/s41586-018-0363-0

Figure Lengend Snippet: Oligonucleotide primers and siRNAs used in the study

Article Snippet: The following antibodies were obtained commercially: mouse anti-ADAR1 mAb (sc-73408, Santa Cruz), rabbit anti-PNPT1 (ab96176, Abcam; sc-49315, Santa Cruz), mouse anti-dsRNA mAb J2 (10010500, Scions), anti-DNA (61014, Progen), rabbit anti-SUV3 (A303-055A, Bethyl Laboratories) 11 , mouse anti-RIG-I mAb (Alme-1) (AG-20B-0009, AdipoGen), rabbit anti-COX IV (3E11, Cell Signaling), rabbit anti-cytochrome c (NB100-91732, Novus Biologicals), rabbit anti-calnexin (2433, Cell Signaling), mouse anti-lamin A/C (4C11, Cell Signaling), donkey anti-mouse IgG (H+L) conjugated with Alexa Fluor 488 (A-21202, Lifetech), goat anti-mouse IgM conjugated with Alexa Fluor 555 (A-21426, Thermo Fisher Scientific), normal mouse IgG2a (sc-3878, Santa Cruz), mouse anti-MDA5 (in house from J.R.), rabbit anti-MAVS (ALX-210-929-C100, Enzo Life Sciences), rabbit anti-Bax (2772T, Cell Signaling), rabbit anti-Bak (6947T, Cell Signaling), rabbit anti-OXA1L (HPA003531, Sigma), mouse anti-α-Tubulin (T5168, Sigma), mouse anti-actin (ab8226, Abcam; A5441, Sigma), rabbit anti-Flag (PA1-984B, Thermo Fisher Scientific), HRP secondary anti-mouse (ab6728, Abcam; A9044, Sigma) 11 , HRP secondary anti-rabbit (A0545, Sigma; ab6721, Abcam) 11 , goat anti-mouse IgG (20 nm gold) preadsorbed (ab27242, Abcam).

Techniques: Sequencing, Luciferase